Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs during fungus infection of soybean (Glycine max L.) roots or elicitor treatment of soybean cell cultures at the onset of phytoalexin synthesis.
Identifieur interne : 002D44 ( Main/Exploration ); précédent : 002D43; suivant : 002D45Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs during fungus infection of soybean (Glycine max L.) roots or elicitor treatment of soybean cell cultures at the onset of phytoalexin synthesis.
Auteurs : H. Habereder [Allemagne] ; G. Schröder ; J. EbelSource :
- Planta [ 0032-0935 ] ; 1989.
Abstract
The differential regulation of the activities and amounts of mRNAs for two enzymes involved in isoflavonoid phytoalexin biosynthesis in soybean was studied during the early stages after inoculation of primary roots with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of Phytophthora megasperma f.sp. glycinea, the causal fungus of root rot disease. In the incompatible interaction, cloned cDNAs were used to demonstrate that the amounts of phenylalanine ammonia-lyase and chalcone synthase mRNAs increased rapidly at the time of penetration of fungal germ tubes into epidermal cell layers (1-2 h after inoculation) concomitant with the onset of phytoalxxin accumulation; highest levels were reached after about 7 h. In the compatible interaction, only a slight early enhancement of mRNA levels was found and no further increase occurred until about 9 h after inoculation. The time course for changes in the activity of chalcone synthase mRNA also showed major differences between the incompatible and compatible interaction. The observed kinetics for the stimulation of mRNA expression related to phytoalexin synthesis in soybean roots lends further support to the hypothesis that phytoalexin production is an early defense response in the incompatible plant-fungus interaction. The kinetics for the enhancement of mRNA expression after treatment of soybean cell suspension cultures with a glucan elicitor derived from P. megasperma cell walls was similar to that measured during the early stages of the resistant response of soybean roots.
DOI: 10.1007/BF00392154
PubMed: 24212272
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Habereder</name><affiliation wicri:level="3"><nlm:affiliation>Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II der Universität, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II der Universität, Schänzlestrasse 1, D-7800, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Schroder, G" sort="Schroder, G" uniqKey="Schroder G" first="G" last="Schröder">G. Schröder</name></author><author><name sortKey="Ebel, J" sort="Ebel, J" uniqKey="Ebel J" first="J" last="Ebel">J. 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Habereder</name><affiliation wicri:level="3"><nlm:affiliation>Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II der Universität, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II der Universität, Schänzlestrasse 1, D-7800, Freiburg</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Schroder, G" sort="Schroder, G" uniqKey="Schroder G" first="G" last="Schröder">G. Schröder</name></author><author><name sortKey="Ebel, J" sort="Ebel, J" uniqKey="Ebel J" first="J" last="Ebel">J. Ebel</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="1989" type="published">1989</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The differential regulation of the activities and amounts of mRNAs for two enzymes involved in isoflavonoid phytoalexin biosynthesis in soybean was studied during the early stages after inoculation of primary roots with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of Phytophthora megasperma f.sp. glycinea, the causal fungus of root rot disease. In the incompatible interaction, cloned cDNAs were used to demonstrate that the amounts of phenylalanine ammonia-lyase and chalcone synthase mRNAs increased rapidly at the time of penetration of fungal germ tubes into epidermal cell layers (1-2 h after inoculation) concomitant with the onset of phytoalxxin accumulation; highest levels were reached after about 7 h. In the compatible interaction, only a slight early enhancement of mRNA levels was found and no further increase occurred until about 9 h after inoculation. The time course for changes in the activity of chalcone synthase mRNA also showed major differences between the incompatible and compatible interaction. The observed kinetics for the stimulation of mRNA expression related to phytoalexin synthesis in soybean roots lends further support to the hypothesis that phytoalexin production is an early defense response in the incompatible plant-fungus interaction. The kinetics for the enhancement of mRNA expression after treatment of soybean cell suspension cultures with a glucan elicitor derived from P. megasperma cell walls was similar to that measured during the early stages of the resistant response of soybean roots. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24212272</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>177</Volume><Issue>1</Issue><PubDate><Year>1989</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs during fungus infection of soybean (Glycine max L.) roots or elicitor treatment of soybean cell cultures at the onset of phytoalexin synthesis.</ArticleTitle><Pagination><MedlinePgn>58-65</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF00392154</ELocationID><Abstract><AbstractText>The differential regulation of the activities and amounts of mRNAs for two enzymes involved in isoflavonoid phytoalexin biosynthesis in soybean was studied during the early stages after inoculation of primary roots with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of Phytophthora megasperma f.sp. glycinea, the causal fungus of root rot disease. In the incompatible interaction, cloned cDNAs were used to demonstrate that the amounts of phenylalanine ammonia-lyase and chalcone synthase mRNAs increased rapidly at the time of penetration of fungal germ tubes into epidermal cell layers (1-2 h after inoculation) concomitant with the onset of phytoalxxin accumulation; highest levels were reached after about 7 h. In the compatible interaction, only a slight early enhancement of mRNA levels was found and no further increase occurred until about 9 h after inoculation. The time course for changes in the activity of chalcone synthase mRNA also showed major differences between the incompatible and compatible interaction. The observed kinetics for the stimulation of mRNA expression related to phytoalexin synthesis in soybean roots lends further support to the hypothesis that phytoalexin production is an early defense response in the incompatible plant-fungus interaction. The kinetics for the enhancement of mRNA expression after treatment of soybean cell suspension cultures with a glucan elicitor derived from P. megasperma cell walls was similar to that measured during the early stages of the resistant response of soybean roots. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Habereder</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Lehrstuhl für Biochemie der Pflanzen, Institut für Biologie II der Universität, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schröder</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Ebel</LastName><ForeName>J</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal 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sort="Ebel, J" uniqKey="Ebel J" first="J" last="Ebel">J. Ebel</name><name sortKey="Schroder, G" sort="Schroder, G" uniqKey="Schroder G" first="G" last="Schröder">G. Schröder</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Habereder, H" sort="Habereder, H" uniqKey="Habereder H" first="H" last="Habereder">H. Habereder</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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